• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    A multi-dose pipette which comprises a body constituting the handle portion, a cylinder portion or piston chamber with a tip tube, fitted at the bottom end of the said body, a main piston fitted into the cylinder portion or piston chamber. The main piston can be pressed down by means of a press knob and a piston rod against the spring force of a spring and is by a spring returned to the upper position. In addition to the main piston, the pipette also comprises a dose piston with its piston chamber. The maximum stroke volume of the main piston is essentially larger than the stroke volume of the dose piston. The piston chamber of the main piston and the dose piston are connected to each other by means of a channel. The end of the piston chamber of the dose piston facing towards the piston rod is provided with a valve which is fitted so that it opens itself and admits air through the valve into the piston chamber at the upper position of the dose piston. The other end of the piston chamber of the dose piston is provided with a counter-valve which is fitted so that it opens itself and admits air through the valve from the piston chamber of the dose valve into the piston chamber of the main piston at the lower position of the dose piston. By means of repeated movements of the dose piston it is possible to pump precise doses of air into the piston chamber of the main piston. Correspondingly, subsequent liquid quantities of equal relative magnitude escape from the tip vessel.
    公开号:SU1088650A3
    申请号:SU802877702
    申请日:1980-01-22
    公开日:1984-04-23
    发明作者:Тервамяки Юкка
    申请人:Коммандииттиихтие Финнпипетте Осмо А.Суованиеми (Фирма)；
    IPC主号:
    专利说明:

    The invention relates to multi-dose pipette designs and can be used in laboratory practice to suck large quantities of liquid, for example, a reagent into an existing tip, as well as to quickly and easily dispense a liquid in the form of separate sequential doses into test tubes.
    A multi-dose pipette is known, comprising a vertical body with a handle in which a piston chamber is made, a tube for connecting exchangeable tips, which is a continuation of the body, a main spring-loaded piston placed in a piston chamber, a push button, a piston rod that connects the push button with the main a piston, a metering chamber made in the housing coaxially with the piston chamber, and one pit placed in the metering chamber, and the volume of the main piston chamber is 2-50 times, preferably 10-25 times more than the volume I meter ClJ.
    A disadvantage of the known pipette is the low accuracy of the taken doses of the liquid, due to the lack of automatic control during dispensing.
    The aim of the invention is to automatically adjust the dispensed doses of liquid.
    The goal is achieved by the fact that a multi-dose pipette containing a vertical body with a handle, in which a piston chamber is made, a tube for connecting interchangeable tips, is a continuation of the body, a main spring-loaded piston placed in a piston chamber, a push button, a piston rod connecting the pressure piston a button with a main piston, a metering chamber, made in the housing, a piston chamber built. and the piston measured in the metering chamber, and the volume of the main piston chamber is 2-50 times, preferably 10-25 times larger than the volume of the metering chamber, equipped with check valves placed in the upper and lower parts of the piston chamber; a shaped connecting channel connecting the dosing and piston chambers to each other, while in the housing the eccentric piston and dosing chambers are made in parallel with a cylinder located in them, in which the dosing piston is located, and the valve chamber with in it, a check valve is made coaxially with an additional camera.
    In this case, the metering piston is packed with a calibration screw inserted into its end face.
    Moreover, a check valve installed in the upper part of the dispensing chamber is provided with a sealing ring placed in a horizontal annular groove formed in the upper part of the main body, and the central part of the porin dosing is made dumbbell-shaped and located inside the sealing ring.
    The pipette is equipped with a graduation adjustment head.
    A threaded hole is made in the housing, and the upper part of the metering piston is made in the form of a screw screwed into the threaded hole.
    In addition, the metering piston is made in the form of a cylindrical tube with holes in its side wall, the sealing ring opposite each other on both sides, and a-dosages: the piston is made with a collar placed under the sealing ring and provided with an annular disk in it outside the sealing ring.
    Fig. 1 shows a general view of a pipette, the first embodiment, a general sectional view; figure 2 is the same, the second variant of the embodiment of FIG. 3 is a metering device, a longitudinal section; figure 4 - section aa on fig.Z; Fig. 5 shows the construction of a valve located above the piston chamber of the metering piston in a multi-dose pipette (according to Fig.; Figures 6 and 7 are other valve variants.
    The multi-dose pipette includes a housing 1, the main piston 2 located in the porous chamber 3, the piston has an o-ring 4 relative to the entrance to the chamber 5, which forms the sleeve part of the tip 6 of the pipette. The spring 7 presses the piston 2 up to the upper stopper 8. In the chamber 9 formed inside the hollow main piston 2, a metering piston 10 is disposed, which is sealed relative to the surface of the main piston 2 by means of a sealing ring 11 and pressed upward by the spring 12. The metering piston 10 is connected with a button 13, a rod 14 provided with a shoulder 15, which acts as a stop for the spring 12 and limits the movement of the head stem 14 upwards when the shoulder 15 finds the stop 17 placed on the handle 16. At the bottom end 18 of the main piston 2 an opening 19 adapted for the passage of air into the chamber 9.
    In addition, the lower end 18 of the main 2 forms a valve body 20 located inside the main piston 2. A spring 21 located in the valve body 20 pushes the locking means 22 against the shoulder 23. The locking means 22 may be a ball or the like. its peculiarity is that when the metering piston 10 moves downwards by pressing the button 13, the TO-lower end of the metering piston 10 can open the valve 24. The upper end of the locking device 22 can project above the collar 23 (FIG. 1), the end face piston 10 has a constriction part which may extend inside the collar 23. When the dispensing piston 10 moves up, valve 25 raspolozhonny the top portion of the main porin 2 .. This opens the valve may consist of hydrochloric narrowed portion 26 on the dosing piston. 10 (FIG. 5, it permits the passage of air in the main piston 2 when jr4acTOK with narrowing 27 allows the ring 11 to be bypassed when the metering piston 10 moves up. Thus, the principle of the valve shown in FIG. 5 is such that by tapering 27 on the metering piston 10 allows passage of air into the dosing chamber 11; when the dosing piston 10 begins to move downward from its upper position, the edge of the tapering section 27 is pressed against the seal 11 and the piston chamber 9 is closed. The same principle of action and is achieved in the embodiment shown in FIG. b, here the metering piston 10 has the shape of a hollow tube, in which holes 28 and 29 are made. In the initial position of the metering plate 10, AIR enters through the opening 29 into the metering piston 10 and from there a chamber 9, which closes when the aperture 29 passes by the seal 11, and the metering piston 10 is pressed downwards. The valve shown in Fig. 7 operates as follows. When the metering piston 10 rises up, the flange 30 formed on it lifts the seal 11, slightly pulling it away from the mating surface 31. When the metering piston 10 moves down, the spring 12 presses the seal 11 to the mating surface 31, most porfnevuyu camera 9. Pipette works as follows. When button 13 is pressed down to the bottom, the main 2 and metering 10 pistons push the amount of air determined by their movement into the existing capacity of the tip 32 located at the end of the pipette tip tube 33. If the tip 32 is lowered into the liquid, and the button 13 slides up, the main piston 2 sucks the liquid 34 into the capacity of the tip 32, this happens until the main piston reaches its upper stop 8. After that, the metering piston 10 starts to move up however, at the first moment the blocking means 22, located in the lower part of the main port 2, immediately closes, so the liquid cannot be more than sucked into the capacity of the tip 32. In opposition to this, the upward movement of the metering piston 10 creates a vacuum e in chamber 9 until the metering door reaches its upper position; therefore, valve 25, located at the upper end of the main piston 2, opens to allow air flow and pressure equalization in chamber 9. After / as the container of the tip 32 is removed From the fluid and placed in a predetermined container, for example, at the entrance to the test tube, you can begin dispensing the fluid 34 by pressing button 13, the subsequent movements are performed only by the metering piston 10. When the metering nojaueHb-10 starts to move downwards, valve 25, located at the upper end of the main piston 2, is first blocked. After that, the metering piston 10 begins to compress the air in the chamber 9, in which the pressure increases. When the pressure reaches a predetermined value, it (this pressure) opens the valve located at the lower end of the main piston 2, and air begins to flow into the main piston chamber 3 through the opening 19. If the spring 21 is strong enough, the blocking means 22 will not open-. with pressure, but when the disruptions of the piston 10 reaches its lower position, the pressure will open the blocking means 22. When the portion of air displaced by the metering piston 10 reaches the main piston chamber 9, it will push the appropriate amount of fluid 34 out of the tip container 32. The ratio of volumes. The air displaced by the main piston 2 as well as the metering piston 10 can be, for example, 1:10 or 1:50 or any other ratio, so that by repeating the movements of the metering piston, the number of doses corresponding to this ratio can be obtained (10 or 50 },
    Thus, the metering piston 10 is an air pump that pumps portions of air into the main piston chamber.
    In order to calibrate the itoa value, the lower end of the metering piston can be equipped with an adjusting screw 35. When the screw 35 is turned away from the metering piston 10, it opens the valve earlier, so the dose becomes less. Otherwise, when screw 35 is rotated, the dose size increases.
    The multi-dose pipette may also have the form shown in Fig. 2, where the metering piston 10 is positioned on the side of the main piston 2. In this case the movement of the button 13 can be made shorter, this may be required for some technicians. In such a case, the nojxusHb 10 metering unit has its own buttons 35 and the piston chamber 9, separated from the main porous 2, valves 24 and 25 are located at the two ends of the metering piston. The air channel 36 passes from the valve body 20 to the main piston chamber 3. Liquid 34 is sucked into the container of the tip 32 by pressing the button 13, and the dosage is carried out using the button 35. In other respects, the operation of this pipette is completely similar to that described.
    By the same adjustment it is possible to install portions of the liquid, selected in the container-tip, as well as dosed. The amount of fluid that is drawn can be adjusted, for example, in accordance with the well-known principle applied in the pipette of the regulating volume, where the stroke of the main piston is regulated. The principle of adjusting the dose amount is shown in FIG. Tapering area. 27 forming valve 25 at the top
    The 5 ends of the dispensing piston 10 are designed in such a way that its length is adjusted with the help of a screw 37. When button 35 is turned up / valve 25 closes later when button 35 is pressed and the dose becomes less. The pin 38 in the groove 39 of the poddn 10 dispenser prevents it from rotating when the button 35 is turned while adjusting the size of the dose. Section 5 of the CU from button 35, you can position the adjustment scale 40, which indicates the size of the dose.
    The principle of air injection can be carried out in various other ways, in particular, changing the valve parts, but the principle of operation of the multi-dose pipette remains unchanged.
    t 17


    0aS.f
    -Jj
    32
    ytfig 2
    - //

    -ten
    Fig 5
    FIG. 7
    fig.c
    权利要求:
    Claims (6)
    [1]
    1. MULTI-DOSE PIPET, containing a vertical housing With a handle in which the piston chamber is made, a tube for connecting replaceable tips, which is a continuation of the housing, a main spring-loaded piston placed in the piston chamber, a push button, a piston rod connecting the push button with the main piston, dispensing a chamber made in the housing coaxial to the piston chamber and a piston located in the metering chamber, and the volume of the main piston chamber is 2-50 times, preferably 10-25 times, more than the volume of the metering chambers s, characterized in that, in order to automatically control the dispensed doses of liquid, it is equipped with check valves located in the upper and lower parts of the piston chamber, and in the housing there is a shaped connection channel connecting the metering and piston chambers to each other, while in the housing it is eccentric The piston and metering chambers are provided with a cylindrical additional chamber located parallel to them, in which the metering piston is located, and the valve chamber with the non-return valve located in it is made sleep under an additional camera.
    [2]
    2. The pipette according to claim 1, characterized in that the metering piston is equipped with a calibration screw screwed into its end.
    [3]
    3. The pipette according to claim 1, with the fact that the “check valve installed in the upper part of the metering chamber” is equipped with a sealing ring placed in a horizontal annular groove made in the upper part of the main piston, and the central part of the metering piston g is made dumbbell-shaped and located inside the o-ring.
    [4]
    4. Pipette on π. 1, characterized in that it is provided with an adjusting head with divisions.
    [5]
    5. The pipette according to claim 1, with the fact that a threaded hole is made in the housing, and the upper part of the metering piston is made in the form of a screw screwed into the threaded hole.
    [6]
    6. The pipette according to claim 1, with a tain and the fact that the metering port is made in the form of a cylindrical tube with holes in its side wall opposite each other on both sides of the o-ring, and the metering piston is made with a shoulder located under the sealing ring, and is equipped with an annular disk mounted in it outside the sealing ring.
    SU.1088650 f
    类似技术:
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    CA2147966A1|1994-05-26|Dosing dispenser
    US3250441A|1966-05-10|Liquid-dispensing apparatus
    US3567398A|1971-03-02|Semi-automatic pipetting and diluter device
    同族专利:
    公开号 | 公开日
    FR2447216B1|1984-02-10|
    FI790209A|1980-07-24|
    FR2447216A1|1980-08-22|
    FI59343B|1981-04-30|
    FI59343C|1981-08-10|
    US4304138A|1981-12-08|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    US3735902A|1971-07-22|1973-05-29|Instrumentation Labor Inc|Dispenser apparatus|
    FR2295404B1|1974-12-16|1979-08-17|Becton Dickinson Co|
    DE2651333C3|1976-11-10|1980-10-16|Walter Sarstedt Kunststoff-Spritzgusswerk, 5223 Nuembrecht|Suction pipette|
    US4096751A|1977-06-15|1978-06-27|Oxford Laboratories Inc.|Hand-held micropipettor with fluid transfer volume adjustment mechanism|US4593837A|1985-03-15|1986-06-10|Eastman Kodak Company|Variable volume pipette|
    EP0351761B1|1988-07-18|1993-09-08|The Perkin-Elmer Corporation|Sample injector|
    WO1992003714A1|1990-08-22|1992-03-05|Drd Diluter Corporation|Pipette|
    WO1992020778A1|1991-05-24|1992-11-26|Kindconi Pty Limited|Biochemical reaction control|
    US5364596A|1992-12-01|1994-11-15|Rainin Instrument Co., Inc.|Manual pipette with plunger velocity governor, home position latch and trigger release|
    DE19735931C2|1997-08-08|2002-02-07|Lerche Dietmar|Piston stroke pipette for volume metering and viscosity measurement|
    DE19743660C1|1997-10-02|1999-02-04|Eppendorf Geraetebau Netheler|Repeating pipette assembly for esp. laboratory use has detachable syringe|
    EP1539352B1|2002-07-23|2009-12-23|Protedyne Corporation|Liquid handling tool having hollow plunger|
    US7093507B2|2003-05-12|2006-08-22|Bel-Art Products, Inc.|Pipette control arrangement|
    US7185551B2|2003-05-22|2007-03-06|Schwartz H Donald|Pipetting module|
    US6805015B1|2003-05-22|2004-10-19|H. Donald Schwartz|Dual resolution syringe|
    WO2009125337A1|2008-04-08|2009-10-15|Socorex Isba S.A.|Joint for device for metering liquids|
    CN105209922B|2013-03-15|2017-06-09|道格拉斯科学有限责任公司|Pipettor is cleaned by formula|
    CN103623878B|2013-12-16|2014-07-23|山东大学齐鲁医院|Liquid-to-gas volumetric liquid transfer device|
    CN110227567A|2019-06-19|2019-09-13|武汉科技大学|A kind of numerical control high temperature liquid-transfering gun|
    法律状态:
    优先权:
    申请号 | 申请日 | 专利标题
    FI790209A|FI59343C|1979-01-23|1979-01-23|FLERDOSPIPETT|
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